INTRODUCTION:
Isothermal amplification of Nucleic acid or DNA is carried
out at steady temperatures as the term ‘Isothermal’ obviates the need for
thermal cyclers. Nucleic acid amplification technologies are used as leading
methods in detecting and analyzing nucleic acids. These are the emerging DNA technologies
in the field of molecular biology. Nucleic Acid Testing promises rapid,
sensitive and specific diagnosis of infectious, inherited and genetic diseases.
NEED FOR ISOTHERMAL AMPLIFICATION TECHNOLOGIES:
Amplification of nucleic acids or DNA helps in detection and
identification of infectious diseases, genetic disorders and other research
purposes. The
most widely used amplification technology is the Polumerase chain reaction (PCR)
but as it requires a thermocycling machine to separate two DNA strands and then
amplify the required fragment, the possibility of amplifying DNA in isothermal
conditions is considered. DNA polymerase replicates DNA with the aid of various
accessory proteins. Recent identification of these proteins has enabled
development of new in vitro isothermal DNA amplification methods, mimicking
these in vivo mechanisms.
Types of isothermal nucleic
acid amplification methods:
1.
Transcription mediated amplification.
2.
Nucleic acid sequence-based amplification.
3.
Signal mediated amplification of RNA
technology.
4.
Strand displacement amplification.
5.
Rolling circle amplification.
6.
Loop-mediated isothermal amplification of DNA.
7.
Isothermal multiple displacement amplification.
8.
Helicase-dependent amplification.
9.
Single primer isothermal amplification.
10.
Circular helicase-dependent amplification.
CURRENT SCENARIO OF INAAT MARKET:
According to a new market research report by
MarketsandMarkets, the global Isothermal nucleic acid amplification
technologies (INAAT) market has been valued over a billion at a CAGR of 17% and
is expected to reach $1.6 billion by 2017. The Isothermal Nucleic Acid Amplification
Technologies Market report would provide an in-depth analysis of the global
INAAT market, its applications, key trends and drivers, assessment of
technological advancements, investment potential, market size and forecasts by
2017. It also provides a comprehensive picture of various types of INAAT platforms
like LAMP (loop-mediated
isothermal amplification), NASBA (nucleic acid sequence based amplification), TMA
(transcription mediated amplification), SPIA (single primer isothermal
amplification), SDA (strand displacement amplification), and HDA (helicase
dependent amplification).
FUTURE
PROSPECTS:
There
are numerous factors which are prompting the growth of INAAT market such as high
incidence of infectious disease, pandemic threats, and emergence of new
infections. Hence this market is primarily driven due to high demands of the
industry and end users. There have been lots of investment activities over the
past two decades, directed towards research and development of these
technologies, attracting interests industry firms and academic institutes. New
developments such as digital INAAT devices, chip based amplification,
multiplexing and detection of pathogens using fluorescent detection systems are
the emerging trends in INAAT market. The next promising players in the INAAT market
are China, India, South Africa and other regions of Asia pacific, Africa and
untapped markets of Europe. The Key Players in this market are Hologic-Gen-Probe,
bioMerieux, BD Diagnostics, Qiagen, Alere, Eiken Chemical, Meridian Bioscience,
BioHelix and Quidel.
The report can be referred here:
http://www.prweb.com/releases/isothermal-nucleic-acid/technologies-inaat-market/prweb10352286.htm
http://www.prweb.com/releases/isothermal-nucleic-acid/technologies-inaat-market/prweb10352286.htm
No comments:
Post a Comment